Effectiveness of Canola Oil as an Alternative Clearing Agent to Xylene in Histological Tissue Processing: A Systematic Review and Meta-Analysis
DOI:
https://doi.org/10.63935/hc76yh48Keywords:
diabetes mellitus, socioeconomic status, blood glucose, social determinants of healthAbstract
Background: Diabetes mellitus is a non-communicable disease with a continuously rising global and national prevalence, and its occurrence is influenced not only by biological factors but also by social determinants such as socioeconomic status, including education, occupation, and income. Purpose: This study aimed to determine the relationship between socioeconomic status and the incidence of diabetes mellitus at Ujungloe Public Health Center. Methods: This quantitative study used an analytical observational approach with a cross-sectional design. The population consisted of 912 patients with diabetes mellitus, from whom 90 respondents were selected using purposive sampling. Data were collected through questionnaires and medical records and analyzed using Spearman's rank correlation test with a significance level of p < 0.05. Results: The results showed a significant relationship between socioeconomic status and the incidence of diabetes mellitus, with p = 0.000 (p < 0.05) and a correlation coefficient of r = 0.809, indicating a very strong positive relationship. Respondents with low socioeconomic status tended to have higher blood glucose levels than those in the middle and high categories. Conclusion: Socioeconomic status is an important determinant of diabetes mellitus incidence through its influence on lifestyle, healthcare access, and health literacy.
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1. Ali, A. H., et al. (2024). Efficacy of eco-friendly clearing agents in tissue processing: A path toward sustainable laboratories. Biomedical Reports, 31(2), 103–112. https://doi.org/10.3892/br.2024.1720
2. Alturkistani, H. A., Tashkandi, F. M., & Mohammedsaleh, Z. M. (2018). Histological stains: A literature review and case study. Global Journal of Health Science, 8(3), 72–79. https://doi.org/10.5539/gjhs.v8n3p72
3. Buesa, R. J., & Peshkov, M. V. (2019). Histology without xylene. Annals of Diagnostic Pathology, 13(4), 246–256. https://doi.org/10.1016/j.anndiagpath.2019.04.004
4. Eshadi, A. M., Gashut, A., & El-Geddawi, A. (2021). Evaluation of canola oil as a safe alternative to xylene in histopathological processing. Libyan Journal of Medical Research, 15(2), 45–53.
5. Gaddikeri, K., Srikant, N., & Radhakrishnan, R. (2022). Eco-friendly alternatives to xylene in hematoxylin and eosin staining: A comparative study. Journal of Oral and Maxillofacial Pathology, 26(1), 118–123. https://doi.org/10.4103/jomfp.jomfp_221_21
6. Gopal, S., et al. (2021). Coconut oil and olive oil as xylene substitutes in histopathology. Journal of Oral Pathology & Medicine, 50(6), 612–618. https://doi.org/10.1111/jop.13155
7. Indu, P., et al. (2018). Efficacy of vegetable oils as an alternative to xylene in tissue processing. Indian Journal of Pathology and Microbiology, 61(2), 188–193. https://doi.org/10.4103/ijpm.ijpm_166_17
8. Kandyala, R., Raghavendra, S. P. C., & Rajasekharan, S. T. (2018). Xylene: An overview of its toxicity and pathways of exposure. Interdisciplinary Toxicology, 3(2), 41–45. https://doi.org/10.2478/v10102-010-0009-4
9. Kurniawan, H. (2018). Penggunaan minyak nabati sebagai clearing agent alternatif dalam mikroteknik. Media Medika Indonesiana, 52(3), 145–152. https://doi.org/10.22146/mgi.31215
10. Nandakumar, S., Joseph, M., & Kumar, A. (2023). Bio-clearing agents: A green approach in histotechnology. Journal of Histotechnology, 46(2), 78–87. https://doi.org/10.1080/01478885.2023.2189021
11. Page, M. J., McKenzie, J. E., Bossuyt, P. M., Boutron, I., Hoffmann, T. C., Mulrow, C. D., Shamseer, L., Tetzlaff, J. M., Akl, E. A., Brennan, S. E., Chou, R., Glanville, J., Grimshaw, J. M., Hróbjartsson, A., Lalu, M. M., Li, T., Loder, E. W., Mayo-Wilson, E., McDonald, S., ... Moher, D. (2021). The PRISMA 2020 statement: An updated guideline for reporting systematic reviews. BMJ, 372(71), 1–9. https://doi.org/10.1136/bmj.n71
12. Premalatha, B. R., et al. (2020). Natural clearing agents: A convincing substitute for xylene. Journal of International Oral Health, 12(4), 312–317. https://doi.org/10.4103/jioh.jioh_250_19
13. Rajan, S., & Kumar, P. (2022). Viscosity and penetration challenges of bio-clearing agents in routine histopathology. International Journal of Pathology Research, 14(3), 201–209.
14. Rasmussen, E., et al. (2016). Vegetable oils as alternate clearing agents in tissue processing. Journal of Histomedicine and Tissue Biomarkers, 8(1), 34–41. https://doi.org/10.1055/s-0038-1647180
15. Raza, M., Akhter, S., & Ali, M. (2021). Xylene-free tissue processing: The quest for an ideal clearing agent. Biomedical Reports, 14(4), 35–42. https://doi.org/10.3892/br.2021.1411
16. Sujathan, S., Sreedhar, S., & Devakanthi, N. (2020). Canola oil: A cost-effective and non-toxic substitute for xylene in histopathology laboratories. Indian Journal of Pathology and Oncology, 7(4), 589–594. https://doi.org/10.18231/j.ijpo.2020.116
17. Swamy, K., et al. (2015). Evaluation of vegetable oils as a substitute for xylene in histopathology. Journal of Clinical and Diagnostic Research, 9(11), EC01–EC04. https://doi.org/10.7860/JCDR/2015/12534.5954
18. Widiastuti, R., & Utami, T. (2025). Analisis sitofisiologi sediaan histopatologi dengan penjernih berbasis minyak asam lemak tidak jenuh. Jurnal Analis Kesehatan, 14(1), 44–51.
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Copyright (c) 2026 Margareth Tumansery, Gabriella Aurel Kindangen (Author)
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